Development of an enzymatic reaction cascade for the production of KDG from seaweed carbohydrates
Abstract In this paper development of a cell-free enzymatic cascade is described for conversion of brown seaweed carbohydrates (alginate and glucose from laminarin) to 2-keto-3-deoxygluconate (KDG), a precursor in bioplastics production. The process involves five enzymatic steps in two parallel pathways (alginate to KDG and glucose to KDG) including alginate depolymerization, cofactor recycling (NADH/NAD+), and … Read more